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2018


Redox metals homeostasis in multiple sclerosis and amyotrophic lateral sclerosis: a review
Redox metals homeostasis in multiple sclerosis and amyotrophic lateral sclerosis: a review

Sheykhansari, S., Kozielski, K., Bill, J., Sitti, M., Gemmati, D., Zamboni, P., Singh, A. V.

Cell Death \& Disease, 9(3):348, March 2018 (article)

Abstract
The effect of redox metals such as iron and copper on multiple sclerosis and amyotrophic lateral sclerosis has been intensively studied. However, the origin of these disorders remains uncertain. This review article critically describes the physiology of redox metals that produce oxidative stress, which in turn leads to cascades of immunomodulatory alteration of neurons in multiple sclerosis and amyotrophic lateral sclerosis. Iron and copper overload has been well established in motor neurons of these diseases' lesions. On the other hand, the role of other metals like cadmium participating indirectly in the redox cascade of neurobiological mechanism is less studied. In the second part of this review, we focus on this less conspicuous correlation between cadmium as an inactive-redox metal and multiple sclerosis and amyotrophic lateral sclerosis, providing novel treatment modalities and approaches as future prospects.

link (url) DOI [BibTex]

2018

link (url) DOI [BibTex]


Cancer cells biomineralize ionic gold into nanoparticles-microplates via secreting defense proteins with specific gold-binding peptides
Cancer cells biomineralize ionic gold into nanoparticles-microplates via secreting defense proteins with specific gold-binding peptides

Singh, A. V., Jahnke, T., Kishore, V., Park, B., Batuwangala, M., Bill, J., Sitti, M.

Acta Biomaterialia, March 2018 (article)

Abstract
Cancer cells have the capacity to synthesize nanoparticles (NPs). The detailed mechanism of this process is not very well documented. We report the mechanism of biomineralization of aqueous gold chloride into NPs and microplates in the breast-cancer cell line MCF7. Spherical gold NPs are synthesized in these cells in the presence of serum in the culture media by the reduction of HAuCl4. In the absence of serum, the cells exhibit gold microplate formation through seed-mediate growth albeit slower reduction. The structural characteristics of the two types of NPs under different media conditions were confirmed using scanning electron microscopy (SEM); crystallinity and metallic properties were assessed with transmission electron microscopy (TEM) and x-ray photoelectron spectroscopy (XPS). Gold-reducing proteins, related to cell stress initiate the biomineralization of HAuCl4 in cells (under serum free conditions) as confirmed by infrared (IR) spectroscopy. MCF7 cells undergo irreversible replicative senescence when exposed to a high concentration of ionic gold and conversely remain in a dormant reversible quiescent state when exposed to a low gold concentration. The latter cellular state was achievable in the presence of the rho/ROCK inhibitor Y-27632. Proteomic analysis revealed consistent expression of specific proteins under serum and serum-free conditions. A high-throughput proteomic approach to screen gold-reducing proteins and peptide sequences was utilized and validated by quartz crystal microbalance with dissipation (QCM-D). Statement of significance Cancer cells are known to synthesize gold nanoparticles and microstructures, which are promising for bioimaging and other therapeutic applications. However, the detailed mechanism of such biomineralization process is not well understood yet. Herein, we demonstrate that cancer cells exposed to gold ions (grown in serum/serum-free conditions) secrete shock and stress-related proteins with specific gold-binding/reducing polypeptides. Cells undergo reversible senescence and can recover normal physiology when treated with the senescence inhibitor depending on culture condition. The use of mammalian cells as microincubators for synthesis of such particles could have potential influence on their uptake and biocompatibility. This study has important implications for in-situ reduction of ionic gold to anisotropic micro-nanostructures that could be used in-vivo clinical applications and tumor photothermal therapy.

link (url) DOI [BibTex]


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Electroelastic modeling of thin-laminated composite plates with surface-bonded piezo-patches using Rayleigh–Ritz method

Gozum, M. M., Aghakhani, A., Serhat, G., Basdogan, I.

Journal of Intelligent Material Systems and Structures, 29(10):2192–2205, March 2018 (article)

Abstract
Laminated composite panels are extensively used in various engineering applications. Piezoelectric transducers can be integrated into such composite structures for a variety of vibration control and energy harvesting applications. Analyzing the structural dynamics of such electromechanical systems requires precise modeling tools which properly consider the coupling between the piezoelectric elements and the laminates. Although previous analytical models in the literature cover vibration analysis of laminated composite plates with fully covered piezoelectric layers, they do not provide a formulation for modeling the piezoelectric patches that partially cover the plate surface. In this study, a methodology for vibration analysis of laminated composite plates with surface-bonded piezo-patches is developed. Rayleigh–Ritz method is used for solving the modal analysis and obtaining the frequency response functions. The developed model includes mass and stiffness contribution of the piezo-patches as well as the two-way electromechanical coupling effect. Moreover, an accelerated method is developed for reducing the computation time of the modal analysis solution. For validations, system-level finite element simulations are performed in ANSYS software. The results show that the developed analytical model can be utilized for accurate and efficient analysis and design of laminated composite plates with surface-bonded piezo-patches.

DOI [BibTex]

DOI [BibTex]


Thermocapillary-driven fluid flow within microchannels
Thermocapillary-driven fluid flow within microchannels

Amador, G. J., Tabak, A. F., Ren, Z., Alapan, Y., Yasa, O., Sitti, M.

ArXiv e-prints, Febuary 2018 (article)

Abstract
Surface tension gradients induce Marangoni flow, which may be exploited for fluid transport. At the micrometer scale, these surface-driven flows can be more significant than those driven by pressure. By introducing fluid-fluid interfaces on the walls of microfluidic channels, we use surface tension gradients to drive bulk fluid flows. The gradients are specifically induced through thermal energy, exploiting the temperature dependence of a fluid-fluid interface to generate thermocapillary flow. In this report, we provide the design concept for a biocompatible, thermocapillary microchannel capable of being powered by solar irradiation. Using temperature gradients on the order of degrees Celsius per centimeter, we achieve fluid velocities on the order of millimeters per second. Following experimental observations, fluid dynamic models, and numerical simulation, we find that the fluid velocity is linearly proportional to the provided temperature gradient, enabling full control of the fluid flow within the microchannels.

link (url) Project Page [BibTex]


Sparse-then-dense alignment-based 3D map reconstruction method for endoscopic capsule robots
Sparse-then-dense alignment-based 3D map reconstruction method for endoscopic capsule robots

Turan, M., Pilavci, Y. Y., Ganiyusufoglu, I., Araujo, H., Konukoglu, E., Sitti, M.

Machine Vision and Applications, 29(2):345-359, Febuary 2018 (article)

Abstract
Despite significant progress achieved in the last decade to convert passive capsule endoscopes to actively controllable robots, robotic capsule endoscopy still has some challenges. In particular, a fully dense three-dimensional (3D) map reconstruction of the explored organ remains an unsolved problem. Such a dense map would help doctors detect the locations and sizes of the diseased areas more reliably, resulting in more accurate diagnoses. In this study, we propose a comprehensive medical 3D reconstruction method for endoscopic capsule robots, which is built in a modular fashion including preprocessing, keyframe selection, sparse-then-dense alignment-based pose estimation, bundle fusion, and shading-based 3D reconstruction. A detailed quantitative analysis is performed using a non-rigid esophagus gastroduodenoscopy simulator, four different endoscopic cameras, a magnetically activated soft capsule robot, a sub-millimeter precise optical motion tracker, and a fine-scale 3D optical scanner, whereas qualitative ex-vivo experiments are performed on a porcine pig stomach. To the best of our knowledge, this study is the first complete endoscopic 3D map reconstruction approach containing all of the necessary functionalities for a therapeutically relevant 3D map reconstruction.

link (url) DOI [BibTex]

link (url) DOI [BibTex]


Independent Actuation of Two-Tailed Microrobots
Independent Actuation of Two-Tailed Microrobots

Khalil, I. S. M., Tabak, A. F., Hamed, Y., Tawakol, M., Klingner, A., Gohary, N. E., Mizaikoff, B., Sitti, M.

IEEE Robotics and Automation Letters, 3(3):1703-1710, Febuary 2018 (article)

Abstract
A soft two-tailed microrobot in low Reynolds number fluids does not achieve forward locomotion by identical tails regardless to its wiggling frequency. If the tails are nonidentical, zero forward locomotion is also observed at specific oscillation frequencies (which we refer to as the reversal frequencies), as the propulsive forces imparted to the fluid by each tail are almost equal in magnitude and opposite in direction. We find distinct reversal frequencies for the two-tailed microrobots based on their tail length ratio. At these frequencies, the microrobot achieves negligible net displacement under the influence of a periodic magnetic field. This observation allows us to fabricate groups of microrobots with tail length ratio of 1.24 ± 0.11, 1.48 ± 0.08, and 1.71 ± 0.09. We demonstrate selective actuation of microrobots based on prior characterization of their reversal frequencies. We also implement simultaneous flagellar propulsion of two microrobots and show that they can be controlled to swim along the same direction and opposite to each other using common periodic magnetic fields. In addition, independent motion control of two microrobots is achieved toward two different reference positions with average steady-state error of 110.1 ± 91.8 μm and 146.9 ± 105.9 μm.

link (url) DOI [BibTex]

link (url) DOI [BibTex]


Recent Advances in Wearable Transdermal Delivery Systems
Recent Advances in Wearable Transdermal Delivery Systems

Amjadi, M., Sheykhansari, S., Nelson, B. J., Sitti, M.

Advanced Materials, 30(7):1704530, January 2018 (article)

Abstract
Abstract Wearable transdermal delivery systems have recently received tremendous attention due to their noninvasive, convenient, and prolonged administration of pharmacological agents. Here, the material prospects, fabrication processes, and drug‐release mechanisms of these types of therapeutic delivery systems are critically reviewed. The latest progress in the development of multifunctional wearable devices capable of closed‐loop sensation and drug delivery is also discussed. This survey reveals that wearable transdermal delivery has already made an impact in diverse healthcare applications, while several grand challenges remain.

link (url) DOI [BibTex]

link (url) DOI [BibTex]


Deep EndoVO: A recurrent convolutional neural network (RCNN) based visual odometry approach for endoscopic capsule robots
Deep EndoVO: A recurrent convolutional neural network (RCNN) based visual odometry approach for endoscopic capsule robots

Turan, M., Almalioglu, Y., Araujo, H., Konukoglu, E., Sitti, M.

Neurocomputing, 275, pages: 1861 - 1870, January 2018 (article)

Abstract
Ingestible wireless capsule endoscopy is an emerging minimally invasive diagnostic technology for inspection of the GI tract and diagnosis of a wide range of diseases and pathologies. Medical device companies and many research groups have recently made substantial progresses in converting passive capsule endoscopes to active capsule robots, enabling more accurate, precise, and intuitive detection of the location and size of the diseased areas. Since a reliable real time pose estimation functionality is crucial for actively controlled endoscopic capsule robots, in this study, we propose a monocular visual odometry (VO) method for endoscopic capsule robot operations. Our method lies on the application of the deep recurrent convolutional neural networks (RCNNs) for the visual odometry task, where convolutional neural networks (CNNs) and recurrent neural networks (RNNs) are used for the feature extraction and inference of dynamics across the frames, respectively. Detailed analyses and evaluations made on a real pig stomach dataset proves that our system achieves high translational and rotational accuracies for different types of endoscopic capsule robot trajectories.

link (url) DOI [BibTex]

link (url) DOI [BibTex]


Small-scale soft-bodied robot with multimodal locomotion
Small-scale soft-bodied robot with multimodal locomotion

Hu, W., Lum, G. Z., Mastrangeli, M., Sitti, M.

Nature, 554, pages: 81-85, Nature, January 2018 (article)

Abstract
Untethered small-scale (from several millimetres down to a few micrometres in all dimensions) robots that can non-invasively access confined, enclosed spaces may enable applications in microfactories such as the construction of tissue scaffolds by robotic assembly1, in bioengineering such as single-cell manipulation and biosensing2, and in healthcare3,4,5,6 such as targeted drug delivery4 and minimally invasive surgery3,5. Existing small-scale robots, however, have very limited mobility because they are unable to negotiate obstacles and changes in texture or material in unstructured environments7,8,9,10,11,12,13. Of these small-scale robots, soft robots have greater potential to realize high mobility via multimodal locomotion, because such machines have higher degrees of freedom than their rigid counterparts14,15,16. Here we demonstrate magneto-elastic soft millimetre-scale robots that can swim inside and on the surface of liquids, climb liquid menisci, roll and walk on solid surfaces, jump over obstacles, and crawl within narrow tunnels. These robots can transit reversibly between different liquid and solid terrains, as well as switch between locomotive modes. They can additionally execute pick-and-place and cargo-release tasks. We also present theoretical models to explain how the robots move. Like the large-scale robots that can be used to study locomotion17, these soft small-scale robots could be used to study soft-bodied locomotion produced by small organisms.

link (url) DOI Project Page [BibTex]


Light‐Driven Janus Hollow Mesoporous TiO2–Au Microswimmers
Light‐Driven Janus Hollow Mesoporous TiO2–Au Microswimmers

Sridhar, V., Park, B., Sitti, M.

Advanced Functional Materials, 28(5):1704902, January 2018 (article)

Abstract
Abstract Light‐driven microswimmers have garnered attention for their potential use in various applications, such as environmental remediation, hydrogen evolution, and targeted drug delivery. Janus hollow mesoporous TiO2/Au (JHP–TiO2–Au) microswimmers with enhanced swimming speeds under low‐intensity ultraviolet (UV) light are presented. The swimmers show enhanced swimming speeds both in presence and absence of H2O2. The microswimmers move due to self‐electrophoresis when UV light is incident on them. There is a threefold increase in speed of JHP–TiO2–Au microswimmers in comparison with Janus solid TiO2/Au (JS–TiO2–Au) microswimmers. This increase in their speed is due to the increase in surface area of the porous swimmers and their hollow structure. These microswimmers are also made steerable by using a thin Co magnetic layer. They can be used in potential environmental applications for active photocatalytic degradation of methylene blue and targeted active drug delivery of an anticancer drug (doxurobicin) in vitro in H2O2 solution. Their increased speed from the presence of a hollow mesoporous structure is beneficial for future potential applications, such as hydrogen evolution, selective heterogeneous photocatalysis, and targeted cargo delivery.

link (url) DOI Project Page [BibTex]


Mechanical Rubbing of Blood Clots Using Helical Robots Under Ultrasound Guidance
Mechanical Rubbing of Blood Clots Using Helical Robots Under Ultrasound Guidance

Khalil, I. S. M., Mahdy, D., Sharkawy, A. E., Moustafa, R. R., Tabak, A. F., Mitwally, M. E., Hesham, S., Hamdi, N., Klingner, A., Mohamed, A., Sitti, M.

IEEE Robotics and Automation Letters, 3(2):1112-1119, January 2018 (article)

Abstract
A simple way to mitigate the potential negative sideeffects associated with chemical lysis of a blood clot is to tear its fibrin network via mechanical rubbing using a helical robot. Here, we achieve mechanical rubbing of blood clots under ultrasound guidance and using external magnetic actuation. Position of the helical robot is determined using ultrasound feedback and used to control its motion toward the clot, whereas the volume of the clots is estimated simultaneously using visual feedback. We characterize the shear modulus and ultimate shear strength of the blood clots to predict their removal rate during rubbing. Our in vitro experiments show the ability to move the helical robot controllably toward clots using ultrasound feedback with average and maximum errors of 0.84 ± 0.41 and 2.15 mm, respectively, and achieve removal rate of -0.614 ± 0.303 mm3/min at room temperature (25 °C) and -0.482 ± 0.23 mm3/min at body temperature (37 °C), under the influence of two rotating dipole fields at frequency of 35 Hz. We also validate the effectiveness of mechanical rubbing by measuring the number of red blood cells and platelets past the clot. Our measurements show that rubbing achieves cell count of (46 ± 10.9) × 104 cell/ml, whereas the count in the absence of rubbing is (2 ± 1.41) × 104 cell/ml, after 40 min.

link (url) DOI [BibTex]

link (url) DOI [BibTex]


Method and device for reversibly attaching a phase changing metal to an object
Method and device for reversibly attaching a phase changing metal to an object

Zhou Ye, G. Z. L. M. S.

US Patent Application US 2018/0021892 A1, January 2018 (patent)

Abstract
A method for reversibly attaching a phase changing metal to an object, the method comprising the steps of: providing a substrate having at least one surface at which the phase changing metal is attached, heating the phase changing metal above a phase changing temperature at which the phase changing metal changes its phase from solid to liquid, bringing the phase changing metal, when the phase changing metal is in the liquid phase or before the phase changing metal is brought into the liquid phase, into contact with the object, permitting the phase changing metal to cool below the phase changing temperature, whereby the phase changing metal becomes solid and the object and the phase changing metal become attached to each other, reheating the phase changing metal above the phase changing temperature to liquefy the phase changing metal, and removing the substrate from the object, with the phase changing metal separating from the object and remaining with the substrate.

US Patent Application Database US Patent Application (PDF) [BibTex]


Method of fabricating a shape-changeable magentic member, method of producing a shape changeable magnetic member and shape changeable magnetic member
Method of fabricating a shape-changeable magentic member, method of producing a shape changeable magnetic member and shape changeable magnetic member

Guo Zhan Lum, Z. Y. M. S.

US Patent Application US 2018/0012693 A1, January 2018 (patent)

Abstract
The present invention relates to a method of fabricating a shape-changeable magnetic member comprising a plurality of segments with each segment being able to be magnetized with a desired magnitude and orientation of magnetization, to a method of producing a shape changeable magnetic member composed of a plurality of segments and to a shape changeable magnetic member.

US Patent Application Database US Patent Application (PDF) [BibTex]


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Enhanced Non-Steady Gliding Performance of the MultiMo-Bat through Optimal Airfoil Configuration and Control Strategy

Kim, H., Woodward, M. A., Sitti, M.

In 2018 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS), pages: 1382-1388, 2018 (inproceedings)

[BibTex]

[BibTex]


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Analysis of Magnetic Interaction in Remotely Controlled Magnetic Devices and Its Application to a Capsule Robot for Drug Delivery

Munoz, F., Alici, G., Zhou, H., Li, W., M. Sitti,

IEEE Transactions on Magnetics, 23(1):298-310, 2018 (article)

[BibTex]

[BibTex]


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Anisotropic Gold Nanostructures: Optimization via in Silico Modeling for Hyperthermia

Singh, A., Jahnke, T., Wang, S., Xiao, Y., Alapan, Y., Kharratian, S., Onbasli, M. C., Kozielski, K., David, H., Richter, G., Bill, J., Laux, P., Luch, A., Sitti, M.

ACS Applied Nano Materials, 1(11):6205-6216, 2018 (article)

[BibTex]

[BibTex]


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Collectives of Spinning Mobile Microrobots for Navigation and Object Manipulation at the Air-Water Interface

Wang, W., Kishore, V., Koens, L., Lauga, E., Sitti, M.

In 2018 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS), pages: 1-9, 2018 (inproceedings)

[BibTex]

[BibTex]


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Incorporation of Terbium into a Microalga Leads to Magnetotactic Swimmers

Santomauro, G., Singh, A., Park, B. W., Mohammadrahimi, M., Erkoc, P., Goering, E., Schütz, G., Sitti, M., Bill, J.

Advanced Biosystems, 2(12):1800039, 2018 (article)

[BibTex]

[BibTex]


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Endo-VMFuseNet: A Deep Visual-Magnetic Sensor Fusion Approach for Endoscopic Capsule Robots

Turan, M., Almalioglu, Y., Gilbert, H. B., Sari, A. E., Soylu, U., Sitti, M.

In 2018 IEEE International Conference on Robotics and Automation (ICRA), pages: 1-7, 2018 (inproceedings)

[BibTex]

[BibTex]


Morphological intelligence counters foot slipping in the desert locust and dynamic robots
Morphological intelligence counters foot slipping in the desert locust and dynamic robots

Woodward, M. A., Sitti, M.

Proceedings of the National Academy of Sciences, 115, pages: E8358-E8367, 2018 (article)

Abstract
During dynamic terrestrial locomotion, animals use complex multifunctional feet to extract friction from the environment. However, whether roboticists assume sufficient surface friction for locomotion or actively compensate for slipping, they use relatively simple point-contact feet. We seek to understand and extract the morphological adaptations of animal feet that contribute to enhancing friction on diverse surfaces, such as the desert locust (Schistocerca gregaria) [Bennet-Clark HC (1975) J Exp Biol 63:53–83], which has both wet adhesive pads and spines. A buckling region in their knee to accommodate slipping [Bayley TG, Sutton GP, Burrows M (2012) J Exp Biol 215:1151–1161], slow nerve conduction velocity (0.5–3 m/s) [Pearson KG, Stein RB, Malhotra SK (1970) J Exp Biol 53:299–316], and an ecological pressure to enhance jumping performance for survival [Hawlena D, Kress H, Dufresne ER, Schmitz OJ (2011) Funct Ecol 25:279–288] further suggest that the locust operates near the limits of its surface friction, but without sufficient time to actively control its feet. Therefore, all surface adaptation must be through passive mechanics (morphological intelligence), which are unknown. Here, we report the slipping behavior, dynamic attachment, passive mechanics, and interplay between the spines and adhesive pads, studied through both biological and robotic experiments, which contribute to the locust’s ability to jump robustly from diverse surfaces. We found slipping to be surface-dependent and common (e.g., wood 1.32 ± 1.19 slips per jump), yet the morphological intelligence of the feet produces a significant chance to reengage the surface (e.g., wood 1.10 ± 1.13 reengagements per jump). Additionally, a discovered noncontact-type jump, further studied robotically, broadens the applicability of the morphological adaptations to both static and dynamic attachment.

DOI Project Page [BibTex]

DOI Project Page [BibTex]


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Endosensorfusion: Particle filtering-based multi-sensory data fusion with switching state-space model for endoscopic capsule robots

Turan, M., Almalioglu, Y., Gilbert, H., Araujo, H., Cemgil, T., Sitti, M.

In 2018 IEEE International Conference on Robotics and Automation (ICRA), pages: 1-8, 2018 (inproceedings)

[BibTex]

[BibTex]


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Three‐dimensional patterning in biomedicine: Importance and applications in neuropharmacology

Singh, A. V., Gharat, T., Batuwangala, M., Park, B. W., Endlein, T., Sitti, M.

Journal of Biomedical Materials Research Part B: Applied Biomaterials, 106(3):1369-1382, 2018 (article)

[BibTex]

[BibTex]


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3D nanoprinted plastic kinoform x-ray optics

Sanli, U. T., Ceylan, H., Bykova, I., Weigand, M., Sitti, M., Schütz, G., Keskinbora, K.

{Advanced Materials}, 30(36), Wiley-VCH, Weinheim, 2018 (article)

DOI [BibTex]

DOI [BibTex]


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Controllable switching between planar and helical flagellar swimming of a soft robotic sperm

Khalil, I. S. M., Tabak, A. F., Seif, M. A., Klingner, A., Sitti, M.

PloS One, 13(11):e0206456, 2018 (article)

[BibTex]

[BibTex]


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Kinetics of orbitally shaken particles constrained to two dimensions

Ipparthi, D., Hageman, T. A. G., Cambier, N., Sitti, M., Dorigo, M., Abelmann, L., Mastrangeli, M.

Physical Review E, 98(4):042137, 2018 (article)

[BibTex]

[BibTex]


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Seed-mediated synthesis of plasmonic gold nanoribbons using cancer cells for hyperthermia applications

Singh, A. V., Alapan, Y., Jahnke, T., Laux, P., Luch, A., Aghakhani, A., Kharratian, S., Onbasli, M. C., Bill, J., Sitti, M.

Journal of Materials Chemistry B, 6(46):7573-7581, 2018 (article)

[BibTex]

[BibTex]

2017


Soft Actuators for Small-Scale Robotics
Soft Actuators for Small-Scale Robotics

Hines, L., Petersen, K., Lum, G. Z., Sitti, M.

Advanced Materials, 2017 (article)

Abstract
This review comprises a detailed survey of ongoing methodologies for soft actuators, highlighting approaches suitable for nanometer- to centimeter-scale robotic applications. Soft robots present a special design challenge in that their actuation and sensing mechanisms are often highly integrated with the robot body and overall functionality. When less than a centimeter, they belong to an even more special subcategory of robots or devices, in that they often lack on-board power, sensing, computation, and control. Soft, active materials are particularly well suited for this task, with a wide range of stimulants and a number of impressive examples, demonstrating large deformations, high motion complexities, and varied multifunctionality. Recent research includes both the development of new materials and composites, as well as novel implementations leveraging the unique properties of soft materials.

DOI [BibTex]


A deep learning based fusion of RGB camera information and magnetic localization information for endoscopic capsule robots
A deep learning based fusion of RGB camera information and magnetic localization information for endoscopic capsule robots

Turan, M., Shabbir, J., Araujo, H., Konukoglu, E., Sitti, M.

International Journal of Intelligent Robotics and Applications, 1(4):442-450, December 2017 (article)

Abstract
A reliable, real time localization functionality is crutial for actively controlled capsule endoscopy robots, which are an emerging, minimally invasive diagnostic and therapeutic technology for the gastrointestinal (GI) tract. In this study, we extend the success of deep learning approaches from various research fields to the problem of sensor fusion for endoscopic capsule robots. We propose a multi-sensor fusion based localization approach which combines endoscopic camera information and magnetic sensor based localization information. The results performed on real pig stomach dataset show that our method achieves sub-millimeter precision for both translational and rotational movements.

link (url) DOI [BibTex]

link (url) DOI [BibTex]


3D Chemical Patterning of Micromaterials for Encoded Functionality
3D Chemical Patterning of Micromaterials for Encoded Functionality

Ceylan, H., Yasa, I. C., Sitti, M.

Advanced Materials, 2017 (article)

Abstract
Programming local chemical properties of microscale soft materials with 3D complex shapes is indispensable for creating sophisticated functionalities, which has not yet been possible with existing methods. Precise spatiotemporal control of two-photon crosslinking is employed as an enabling tool for 3D patterning of microprinted structures for encoding versatile chemical moieties.

DOI Project Page [BibTex]


Biohybrid actuators for robotics: A review of devices actuated by living cells
Biohybrid actuators for robotics: A review of devices actuated by living cells

Ricotti, L., Trimmer, B., Feinberg, A. W., Raman, R., Parker, K. K., Bashir, R., Sitti, M., Martel, S., Dario, P., Menciassi, A.

Science Robotics, 2(12), Science Robotics, November 2017 (article)

Abstract
Actuation is essential for artificial machines to interact with their surrounding environment and to accomplish the functions for which they are designed. Over the past few decades, there has been considerable progress in developing new actuation technologies. However, controlled motion still represents a considerable bottleneck for many applications and hampers the development of advanced robots, especially at small length scales. Nature has solved this problem using molecular motors that, through living cells, are assembled into multiscale ensembles with integrated control systems. These systems can scale force production from piconewtons up to kilonewtons. By leveraging the performance of living cells and tissues and directly interfacing them with artificial components, it should be possible to exploit the intricacy and metabolic efficiency of biological actuation within artificial machines. We provide a survey of important advances in this biohybrid actuation paradigm.

link (url) DOI [BibTex]

link (url) DOI [BibTex]


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Editorial for the Special Issue on Microdevices and Microsystems for Cell Manipulation

Hu, W., Ohta, A. T.

8, Multidisciplinary Digital Publishing Institute, September 2017 (misc)

DOI [BibTex]

DOI [BibTex]


Multifunctional Bacteria-Driven Microswimmers for Targeted Active Drug Delivery
Multifunctional Bacteria-Driven Microswimmers for Targeted Active Drug Delivery

Park, B., Zhuang, J., Yasa, O., Sitti, M.

ACS Nano, 11(9):8910-8923, September 2017, PMID: 28873304 (article)

Abstract
High-performance, multifunctional bacteria-driven microswimmers are introduced using an optimized design and fabrication method for targeted drug delivery applications. These microswimmers are made of mostly single Escherichia coli bacterium attached to the surface of drug-loaded polyelectrolyte multilayer (PEM) microparticles with embedded magnetic nanoparticles. The PEM drug carriers are 1 μm in diameter and are intentionally fabricated with a more viscoelastic material than the particles previously studied in the literature. The resulting stochastic microswimmers are able to swim at mean speeds of up to 22.5 μm/s. They can be guided and targeted to specific cells, because they exhibit biased and directional motion under a chemoattractant gradient and a magnetic field, respectively. Moreover, we demonstrate the microswimmers delivering doxorubicin anticancer drug molecules, encapsulated in the polyelectrolyte multilayers, to 4T1 breast cancer cells under magnetic guidance in vitro. The results reveal the feasibility of using these active multifunctional bacteria-driven microswimmers to perform targeted drug delivery with significantly enhanced drug transfer, when compared with the passive PEM microparticles.

link (url) DOI Project Page [BibTex]


EndoSensorFusion: Particle Filtering-Based Multi-sensory Data Fusion with Switching State-Space Model for Endoscopic Capsule Robots
EndoSensorFusion: Particle Filtering-Based Multi-sensory Data Fusion with Switching State-Space Model for Endoscopic Capsule Robots

Turan, M., Almalioglu, Y., Gilbert, H., Araujo, H., Cemgil, T., Sitti, M.

ArXiv e-prints, September 2017 (article)

Abstract
A reliable, real time multi-sensor fusion functionality is crucial for localization of actively controlled capsule endoscopy robots, which are an emerging, minimally invasive diagnostic and therapeutic technology for the gastrointestinal (GI) tract. In this study, we propose a novel multi-sensor fusion approach based on a particle filter that incorporates an online estimation of sensor reliability and a non-linear kinematic model learned by a recurrent neural network. Our method sequentially estimates the true robot pose from noisy pose observations delivered by multiple sensors. We experimentally test the method using 5 degree-of-freedom (5-DoF) absolute pose measurement by a magnetic localization system and a 6-DoF relative pose measurement by visual odometry. In addition, the proposed method is capable of detecting and handling sensor failures by ignoring corrupted data, providing the robustness expected of a medical device. Detailed analyses and evaluations are presented using ex-vivo experiments on a porcine stomach model prove that our system achieves high translational and rotational accuracies for different types of endoscopic capsule robot trajectories.

link (url) Project Page [BibTex]


Endo-VMFuseNet: Deep Visual-Magnetic Sensor Fusion Approach for Uncalibrated, Unsynchronized and Asymmetric Endoscopic Capsule Robot Localization Data
Endo-VMFuseNet: Deep Visual-Magnetic Sensor Fusion Approach for Uncalibrated, Unsynchronized and Asymmetric Endoscopic Capsule Robot Localization Data

Turan, M., Almalioglu, Y., Gilbert, H., Eren Sari, A., Soylu, U., Sitti, M.

ArXiv e-prints, September 2017 (article)

Abstract
In the last decade, researchers and medical device companies have made major advances towards transforming passive capsule endoscopes into active medical robots. One of the major challenges is to endow capsule robots with accurate perception of the environment inside the human body, which will provide necessary information and enable improved medical procedures. We extend the success of deep learning approaches from various research fields to the problem of uncalibrated, asynchronous, and asymmetric sensor fusion for endoscopic capsule robots. The results performed on real pig stomach datasets show that our method achieves sub-millimeter precision for both translational and rotational movements and contains various advantages over traditional sensor fusion techniques.

link (url) Project Page [BibTex]


Magnetotactic Bacteria Powered Biohybrids Target E. coli Biofilms
Magnetotactic Bacteria Powered Biohybrids Target E. coli Biofilms

Stanton, M. M., Park, B., Vilela, D., Bente, K., Faivre, D., Sitti, M., Sánchez, S.

ACS Nano, 0(0):null, September 2017, PMID: 28933815 (article)

Abstract
Biofilm colonies are typically resistant to general antibiotic treatment and require targeted methods for their removal. One of these methods includes the use of nanoparticles as carriers for antibiotic delivery, where they randomly circulate in fluid until they make contact with the infected areas. However, the required proximity of the particles to the biofilm results in only moderate efficacy. We demonstrate here that the nonpathogenic magnetotactic bacteria Magnetosopirrillum gryphiswalense (MSR-1) can be integrated with drug-loaded mesoporous silica microtubes to build controllable microswimmers (biohybrids) capable of antibiotic delivery to target an infectious biofilm. Applying external magnetic guidance capability and swimming power of the MSR-1 cells, the biohybrids are directed to and forcefully pushed into matured Escherichia coli (E. coli) biofilms. Release of the antibiotic, ciprofloxacin, is triggered by the acidic microenvironment of the biofilm, ensuring an efficient drug delivery system. The results reveal the capabilities of a nonpathogenic bacteria species to target and dismantle harmful biofilms, indicating biohybrid systems have great potential for antibiofilm applications.

link (url) DOI Project Page [BibTex]

link (url) DOI Project Page [BibTex]


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Swimming in low reynolds numbers using planar and helical flagellar waves

Khalil, I. S. M., Tabak, A. F., Seif, M. A., Klingner, A., Adel, B., Sitti, M.

In International Conference on Intelligent Robots and Systems (IROS) 2017, pages: 1907-1912, International Conference on Intelligent Robots and Systems, September 2017 (inproceedings)

Abstract
In travelling towards the oviducts, sperm cells undergo transitions between planar to helical flagellar propulsion by a beating tail based on the viscosity of the environment. In this work, we aim to model and mimic this behaviour in low Reynolds number fluids using externally actuated soft robotic sperms. We numerically investigate the effects of transition between planar to helical flagellar propulsion on the swimming characteristics of the robotic sperm using a model based on resistive-force theory to study the role of viscous forces on its flexible tail. Experimental results are obtained using robots that contain magnetic particles within the polymer matrix of its head and an ultra-thin flexible tail. The planar and helical flagellar propulsion are achieved using in-plane and out-of-plane uniform fields with sinusoidally varying components, respectively. We experimentally show that the swimming speed of the robotic sperm increases by a factor of 1.4 (fluid viscosity 5 Pa.s) when it undergoes a controlled transition between planar to helical flagellar propulsion, at relatively low actuation frequencies.

DOI [BibTex]

DOI [BibTex]


Sparse-then-Dense Alignment based 3D Map Reconstruction Method for Endoscopic Capsule Robots
Sparse-then-Dense Alignment based 3D Map Reconstruction Method for Endoscopic Capsule Robots

Turan, M., Yigit Pilavci, Y., Ganiyusufoglu, I., Araujo, H., Konukoglu, E., Sitti, M.

ArXiv e-prints, August 2017 (article)

Abstract
Since the development of capsule endoscopcy technology, substantial progress were made in converting passive capsule endoscopes to robotic active capsule endoscopes which can be controlled by the doctor. However, robotic capsule endoscopy still has some challenges. In particular, the use of such devices to generate a precise and globally consistent three-dimensional (3D) map of the entire inner organ remains an unsolved problem. Such global 3D maps of inner organs would help doctors to detect the location and size of diseased areas more accurately, precisely, and intuitively, thus permitting more accurate and intuitive diagnoses. The proposed 3D reconstruction system is built in a modular fashion including preprocessing, frame stitching, and shading-based 3D reconstruction modules. We propose an efficient scheme to automatically select the key frames out of the huge quantity of raw endoscopic images. Together with a bundle fusion approach that aligns all the selected key frames jointly in a globally consistent way, a significant improvement of the mosaic and 3D map accuracy was reached. To the best of our knowledge, this framework is the first complete pipeline for an endoscopic capsule robot based 3D map reconstruction containing all of the necessary steps for a reliable and accurate endoscopic 3D map. For the qualitative evaluations, a real pig stomach is employed. Moreover, for the first time in literature, a detailed and comprehensive quantitative analysis of each proposed pipeline modules is performed using a non-rigid esophagus gastro duodenoscopy simulator, four different endoscopic cameras, a magnetically activated soft capsule robot (MASCE), a sub-millimeter precise optical motion tracker and a fine-scale 3D optical scanner.

link (url) Project Page [BibTex]

link (url) Project Page [BibTex]


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Dipole codes attractively encode glue functions

Ipparthi, D., Mastrangeli, M., Winslow, A.

Theoretical Computer Science, 671, pages: 19 - 25, August 2017, Computational Self-Assembly (article)

Abstract
Dipole words are sequences of magnetic dipoles, in which alike elements repel and opposite elements attract. Magnetic dipoles contrast with more general sets of bonding types, called glues, in which pairwise bonding strength is specified by a glue function. We prove that every glue function g has a set of dipole words, called a dipole code, that attractively encodes g: the pairwise attractions (positive or non-positive bond strength) between the words are identical to those of g. Moreover, we give such word sets of asymptotically optimal length. Similar results are obtained for a commonly used subclass of glue functions.

link (url) DOI [BibTex]

link (url) DOI [BibTex]


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Hypoxia‐enhanced adhesion of red blood cells in microscale flow

Kim, M., Alapan, Y., Adhikari, A., Little, J. A., Gurkan, U. A.

Microcirculation, 24(5):e12374, July 2017 (article)

Abstract
Abstract Objectives The advancement of microfluidic technology has facilitated the simulation of physiological conditions of the microcirculation, such as oxygen tension, fluid flow, and shear stress in these devices. Here, we present a micro‐gas exchanger integrated with microfluidics to study RBC adhesion under hypoxic flow conditions mimicking postcapillary venules. Methods We simulated a range of physiological conditions and explored RBC adhesion to endothelial or subendothelial components (FN or LN). Blood samples were injected into microchannels at normoxic or hypoxic physiological flow conditions. Quantitative evaluation of RBC adhesion was performed on 35 subjects with homozygous SCD. Results Significant heterogeneity in RBC adherence response to hypoxia was seen among SCD patients. RBCs from a HEA population showed a significantly greater increase in adhesion compared to RBCs from a HNA population, for both FN and LN. Conclusions The approach presented here enabled the control of oxygen tension in blood during microscale flow and the quantification of RBC adhesion in a cost‐efficient and patient‐specific manner. We identified a unique patient population in which RBCs showed enhanced adhesion in hypoxia in vitro. Clinical correlates suggest a more severe clinical phenotype in this subgroup.

link (url) DOI [BibTex]

link (url) DOI [BibTex]


An XY ϴz flexure mechanism with optimal stiffness properties
An XY ϴz flexure mechanism with optimal stiffness properties

Lum, G. Z., Pham, M. T., Teo, T. J., Yang, G., Yeo, S. H., Sitti, M.

In 2017 IEEE International Conference on Advanced Intelligent Mechatronics (AIM), pages: 1103-1110, July 2017 (inproceedings)

Abstract
The development of optimal XY θz flexure mechanisms, which can deliver high precision motion about the z-axis, and along the x- and y-axes is highly desirable for a wide range of micro/nano-positioning tasks pertaining to biomedical research, microscopy technologies and various industrial applications. Although maximizing the stiffness ratios is a very critical design requirement, the achievable translational and rotational stiffness ratios of existing XY θz flexure mechanisms are still restricted between 0.5 and 130. As a result, these XY θz flexure mechanisms are unable to fully optimize their workspace and capabilities to reject disturbances. Here, we present an optimal XY θz flexure mechanism, which is designed to have maximum stiffness ratios. Based on finite element analysis (FEA), it has translational stiffness ratio of 248, rotational stiffness ratio of 238 and a large workspace of 2.50 mm × 2.50 mm × 10°. Despite having such a large workspace, FEA also predicts that the proposed mechanism can still achieve a high bandwidth of 70 Hz. In comparison, the bandwidth of similar existing flexure mechanisms that can deflect more than 0.5 mm or 0.5° is typically less than 45 Hz. Hence, the high stiffness ratios of the proposed mechanism are achieved without compromising its dynamic performance. Preliminary experimental results pertaining to the mechanism's translational actuating stiffness and bandwidth were in agreement with the FEA predictions as the deviation was within 10%. In conclusion, the proposed flexure mechanism exhibits superior performance and can be used across a wide range of applications.

DOI [BibTex]

DOI [BibTex]


Positioning of drug carriers using permanent magnet-based robotic system in three-dimensional space
Positioning of drug carriers using permanent magnet-based robotic system in three-dimensional space

Khalil, I. S. M., Alfar, A., Tabak, A. F., Klingner, A., Stramigioli, S., Sitti, M.

In 2017 IEEE International Conference on Advanced Intelligent Mechatronics (AIM), pages: 1117-1122, July 2017 (inproceedings)

Abstract
Magnetic control of drug carriers using systems with open-configurations is essential to enable scaling to the size of in vivo applications. In this study, we demonstrate motion control of paramagnetic microparticles in a low Reynolds number fluid, using a permanent magnet-based robotic system with an open-configuration. The microparticles are controlled in three-dimensional (3D) space using a cylindrical NdFeB magnet that is fixed to the end-effector of a robotic arm. We develop a kinematic map between the position of the microparticles and the configuration of the robotic arm, and use this map as a basis of a closed-loop control system based on the position of the microparticles. Our experimental results show the ability of the robot configuration to control the exerted field gradient on the dipole of the microparticles, and achieve positioning in 3D space with maximum error of 300 µm and 600 µm in the steady-state during setpoint and trajectory tracking, respectively.

DOI [BibTex]

DOI [BibTex]


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Self-assembly of micro/nanosystems across scales and interfaces

Mastrangeli, M.

In 2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), pages: 676 - 681, IEEE, July 2017 (inproceedings)

Abstract
Steady progress in understanding and implementation are establishing self-assembly as a versatile, parallel and scalable approach to the fabrication of transducers. In this contribution, I illustrate the principles and reach of self-assembly with three applications at different scales - namely, the capillary self-alignment of millimetric components, the sealing of liquid-filled polymeric microcapsules, and the accurate capillary assembly of single nanoparticles - and propose foreseeable directions for further developments.

link (url) DOI [BibTex]

link (url) DOI [BibTex]


Dynamic analysis on hexapedal water-running robot with compliant joints
Dynamic analysis on hexapedal water-running robot with compliant joints

Kim, H., Liu, Y., Jeong, K., Sitti, M., Seo, T.

In 2017 14th International Conference on Ubiquitous Robots and Ambient Intelligence (URAI), pages: 250-251, June 2017 (inproceedings)

Abstract
The dynamic analysis has been considered as one of the important design methods to design robots. In this research, we derive dynamic equation of hexapedal water-running robot to design compliant joints. The compliant joints that connect three bodies will be used to improve mobility and stability of water-running motion's pitch behavior. We considered all of parts as rigid body including links of six Klann mechanisms and three main frames. And then, we derived dynamic equation by using the Lagrangian method with external force of the water. We are expecting that the dynamic analysis is going to be used to design parts of the water running robot.

DOI [BibTex]

DOI [BibTex]


Soiled adhesive pads shear clean by slipping: a robust self-cleaning mechanism in climbing beetles
Soiled adhesive pads shear clean by slipping: a robust self-cleaning mechanism in climbing beetles

Amador, G., Endlein, T., Sitti, M.

Journal of The Royal Society Interface, 14(131):20170134, The Royal Society, June 2017 (article)

Abstract
Animals using adhesive pads to climb smooth surfaces face the problem of keeping their pads clean and functional. Here, a self-cleaning mechanism is proposed whereby soiled feet would slip on the surface due to a lack of adhesion but shed particles in return. Our study offers an in situ quantification of self-cleaning performance in fibrillar adhesives, using the dock beetle as a model organism. After beetles soiled their pads by stepping into patches of spherical beads, we found that their gait was significantly affected. Specifically, soiled pads slipped 10 times further than clean pads, with more particles deposited for longer slips. Like previous studies, we found that particle size affected cleaning performance. Large (45 μm) beads were removed most effectively, followed by medium (10 μm) and small (1 μm). Consistent with our results from climbing beetles, force measurements on freshly severed legs revealed larger detachment forces of medium particles from adhesive pads compared to a flat surface, possibly due to interlocking between fibres. By contrast, dock leaves showed an overall larger affinity to the beads and thus reduced the need for cleaning. Self-cleaning through slippage provides a mechanism robust to particle size and may inspire solutions for artificial adhesives.

DOI Project Page [BibTex]

DOI Project Page [BibTex]


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Yield prediction in parallel homogeneous assembly

Ipparthi, D., Winslow, A., Sitti, M., Dorigo, M., Mastrangeli, M.

Soft Matter, 13, pages: 7595-7608, The Royal Society of Chemistry, June 2017 (article)

Abstract
We investigate the parallel assembly of two-dimensional{,} geometrically-closed modular target structures out of homogeneous sets of macroscopic components of varying anisotropy. The yield predicted by a chemical reaction network (CRN)-based model is quantitatively shown to reproduce experimental results over a large set of conditions. Scaling laws for parallel assembling systems are then derived from the model. By extending the validity of the CRN-based modelling{,} this work prompts analysis and solutions to the incompatible substructure problem.

link (url) DOI [BibTex]

link (url) DOI [BibTex]


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Three‐dimensional patterning in biomedicine: Importance and applications in neuropharmacology

Ajay, V. S., Tanmay, G., Madu, B., Byung‐Wook, P., Thomas, E., Metin, S.

Journal of Biomedical Materials Research Part B: Applied Biomaterials, 106(3):1369-1382, June 2017 (article)

Abstract
Abstract Nature manufactures biological systems in three dimensions with precisely controlled spatiotemporal profiles on hierarchical length and time scales. In this article, we review 3D patterning of biological systems on synthetic platforms for neuropharmacological applications. We briefly describe 3D versus 2D chemical and topographical patterning methods and their limitations. Subsequently, an overview of introducing a third dimension in neuropharmacological research with delineation of chemical and topographical roles is presented. Finally, toward the end of this article, an explanation of how 3D patterning has played a pivotal role in relevant fields of neuropharmacology to understand neurophysiology during development, normal health, and disease conditions is described. The future prospects of organs‐on‐a‐‐like devices to mimic patterned blood–brain barrier in the context of neurotherapeutic discovery and development for the prioritization of lead candidates, membrane potential, and toxicity testing are also described. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1369–1382, 2018.

link (url) DOI [BibTex]

link (url) DOI [BibTex]


Tail-Assisted Mobility and Stability Enhancement in Yaw/Pitch Motions of a Water-Running Robot
Tail-Assisted Mobility and Stability Enhancement in Yaw/Pitch Motions of a Water-Running Robot

Kim, H., Sitti, M., Seo, T.

IEEE/ASME Transactions on Mechatronics, 22(3):1207–1217, IEEE, June 2017 (article)

Abstract
Water-running robots have been developed inspired by a basilisk lizard, which demonstrates highly agile, stable, and energy-efficient locomotion on water surfaces. Current water-running robots are not as stable and agile as their biological counterparts. This study shows how the stability of a water-running robot in the pitch direction can be improved by using an active tail to enable increased propulsion. The mobility of the robot is also increased. To generate force in the pitch and yaw directions, a two-degrees-of-freedom tail is implemented with two circular plates to provide drag. We developed two types of dynamic models for pitch and yaw behavior, and the results are recursively calculated by considering the correlation between the models. The relationship between pitch motion and propulsion was analyzed by simulations. The steering behavior of the robot is also validated while considering the pitch behavior. Experiments were conducted to verify the simulation results.

DOI [BibTex]

DOI [BibTex]


Design and actuation of a magnetic millirobot under a constant unidirectional magnetic field
Design and actuation of a magnetic millirobot under a constant unidirectional magnetic field

Erin, O., Giltinan, J., Tsai, L., Sitti, M.

In 2017 IEEE International Conference on Robotics and Automation (ICRA), Proceedings 2017 IEEE International Conference on Robotics and Automation (ICRA), pages: 3404-3410, IEEE, Piscataway, NJ, USA, IEEE International Conference on Robotics and Automation (ICRA), May 2017 (inproceedings)

Abstract
Magnetic untethered millirobots, which are actuated and controlled by remote magnetic fields, have been proposed for medical applications due to their ability to safely pass through tissues at long ranges. For example, magnetic resonance imaging (MRI) systems with a 3-7 T constant unidirectional magnetic field and 3D gradient coils have been used to actuate magnetic robots. Such magnetically constrained systems place limits on the degrees of freedom that can be actuated for untethered devices. This paper presents a design and actuation methodology for a magnetic millirobot that exhibits both position and orientation control in 2D under a magnetic field, dominated by a constant unidirectional magnetic field as found in MRI systems. Placing a spherical permanent magnet, which is free to rotate inside the millirobot and located away from the center of mass, allows the generation of net forces and torques with applied 3D magnetic field gradients. We model this system in a 3D planar case and experimentally demonstrate open-loop control of both position and orientation by the applied 2D field gradients. The actuation performance is characterized across the most important design variables, and we experimentally demonstrate that the proposed approach is feasible.

DOI [BibTex]

DOI [BibTex]